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Image Search Results
Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Soluble klotho binds monosialoganglioside to regulate membrane microdomains and growth factor signaling
doi: 10.1073/pnas.1620301114
Figure Lengend Snippet: Evidence for klotho down-regulation of lipid raft-associated PI3K signaling in vivo. (A) DAG-stimulated TRPC6 currents were recorded in ventricular myocytes isolated from mice after isoproterenol overstimulation to induce TRPC6 surface expression (9). Data are mean ± SEM of inward current density. n = 10–13 each group. *P < 0.01 vs. control (first bar from left). (B and C) Raft-associated PI3K/Akt signaling (marked by caveolin-3) is up-regulated in klotho-deficient mouse hearts vs. wild-type mouse hearts. Hearts isolated from wild-type or homozygous klotho knockout mice were homogenized, lysed in ice-cold 1% Triton X-100 buffer, and subjected to sucrose gradient ultracentrifugation. B shows one representative experiment. C shows mean ± SEM from four separate experiments. *P < 0.01, wild-type vs. klotho-deficient hearts.
Article Snippet:
Techniques: In Vivo, Isolation, Expressing, Knock-Out